Rapid acetoacetate analysis on the LKB 8600 Reaction Rate Analyser.

Routine determination of 5' nucleotidase activity of human serum using the LKB 8600 reaction rate analyser.

Comparisonof Per - cent of ExpectedValue and Percent Recoveryfor Serum Folate Radioassay

The LKB 8600 Reaction Rate Analyzer sold in the United States, is equipped with a “delay switch,” whereas the same instrument sold in Canada measures time rate reactions immediately after the addition of the initiating reagent, without provision for delay. Therefore, “lag-phases” of any enzyme reaction are recorded with the Canadian version. One of the basics of zero-order kinetic assays is the...

Evaluation of EDTA-chelated bluret reagent containing glycine and sodium chloride.

Table 1. Precision of Lactate and Pyruvatea (1970). Lactate method Pyi’uv.ts method 10. Davidson, D. F., Absorbance accuracy Mean, 50 Mean SO check on the LKB 8600 reaction rate anammol/tftsr CV, % n imol/IItsr CV, % 11 lyzer. J. Clin. Pathol. 29,758(1976). Within-batch precision ii. Bucher, T., Czak, R., Lamprecht, W., and 1.80 0.09 4.9 14 104 4.3 4.1 15 Latzko,E., In Methods of Enzymatic Anal-

Conditions for the estimation of serum lactic dehydrogenase activity using the LKB 3600 reaction rate analyser and the range found in healthy subjects.

A kinetic spectrophotometric assay for rapid determination of acetoacetate in blood.

We describe an automated kinetic assay for acetoacetate in blood. Acetoacetate is enzymatically reduced to D-beta-hydroxybutyrate and the reaction is monitored for 60 s with a reaction-rate analyzer. This technique allows low concentrations of acetoacetate to be measured with good precision and overcomes many of the problems associated with other automated techniques. Our studies on the stabili...